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TECH RESOURCES
Welgen technical specialists are professional researchers with laboratory and product experience. Feel free to contact us with your technical or product inquiry.
Tel: 1-888-493-5436
E-mail: info@welgen.com.

Adenovirus and RNAi Services performed by Welgen Inc. have been directly referenced by the following publications:

Zoltan Arany, Shi-Yin Foo, Yanhong Ma, Jorge L. Ruas1, Archana Bommi-Reddy, Geoffrey Girnun1, Marcus Cooper, Dina Laznik, Jessica Chinsomboon, Shamina M. Rangwala, Kwan Hyuck Baek, Anthony Rosenzweig & Bruce M. Spiegelman. 2008, HIF-independent regulation of VEGF and angiogenesis by the transcriptional coactivator PGC-1a. 2008 Nature 451, 1008-1012.

Jin P, Zhang J, Sumariwalla PF, Ni I, Jorgensen B, Crawford D, Phillips S, Feldmann M, Shepard HM, Paleolog EM. 2008. Novel splice variants derived from the receptor tyrosine kinase superfamily are potential therapeutics for rheumatoid arthritis. 2008 Arthritis Res Ther. Jul 1;10(4):R73. [Epub ahead of print]

Wang B, Haldar SM, Lu Y, Ibrahim OA, Fisch S, Gray S, Leask A, Jain MK. 2008. The Kruppel-like factor KLF15 inhibits connective tissue growth factor (CTGF) expression in cardiac fibroblasts. 2008 J Mol Cell Cardiol. 2008 May 20. [Epub ahead of print]

Guoqing Sheng, Xingshun Xu, Yung-Feng Lin, Chuan-En Wang, Juan Rong, Dongmei Cheng, Junmin Peng, Xiaoyan Jiang, Shi-Hua Li and Xiao-Jiang Li, 2008. Huntingtin-associated protein 1 interacts with Ahi1 to regulate cerebellar and brainstem development in mice. J. Clin. Invest. 10.1172

Kai Chen, Michael T. Kirber, Hui Xiao, Yu Yang, and John F. Keaney, Jr. 2008 Regulation of ROS signal transduction by NADPH oxidase 4 localization. J. Cell Biology, 181:1129-1139.

Gray S, Wang B, Orihuela Y, Hong EG, Fisch S, Haldar S, Cline GW, Kim JK, Peroni OD, Kahn BB, Jain MK. 2007. Regulation of gluconeogenesis by Krüppel-like factor 15. 2007 Cell Metab. 5(4):305-12..

Thirugnana Subramanian and Govindaswamy Chinnadurai. 2007 Temperature Sensitive Replication-Competent Adenovirus shRNA Vectors to Study Cellular Genes in Virus-Induced Apoptosis. 2007 In: Methods in Molecular Medicine, Volume 130, p125-134..

Miyoshi H, Perfield JW 2nd, Souza SC, Shen WJ, Zhang HH, Stancheva ZS, Kraemer FB, Obin MS, Greenberg AS. 2007. Control of adipose triglyceride lipase action by serine 517 of perilipin A globally regulates protein kinase A-stimulated lipolysis in adipocytes. 2007 J Biol Chem. 282(2):996-1002. .

Haiyan Xu, Denise Wilcox, Phong Nguyen, Martin Voorbach, Harriet Smith, Stevan Brodjian, Thomas Suhar, Regina Reilly, Peer Jacobson, Christine A. Collins, Katherine Landschulz, Terry Surowy. 2007. Hepatic knockdown of stearoyl-COA desaturase 1 via RNA interference in obese mice decreases lipid content and changes fatty acid composition. 2007 In: Frontiers in Bioscience 12, 3781-3794.

Sheng G, Chang GQ, Lin JY, Yu ZX, Fang ZH, Rong J, Lipton SA, Li SH, Tong G, Leibowitz SF, Li XJ. 2006. Hypothalamic huntingtin-associated protein 1 as a mediator of feeding behavior. 2006 Nature Medcine. 12:526-33..

Zheng X, Koropatnick J, Li M, Zhang X, Ling F, Ren X, Hao X, Sun H, Vladau C, Franek JA, Feng B, Urquhart BL, Zhong R, Freeman DJ, Garcia B, Min WP. 2006. Reinstalling antitumor immunity by inhibiting tumor-derived immunosuppressive molecule IDO through RNA interference. 2006 J Immunol. 177(8):5639-46..

Hideaki Miyoshi, Sandra C. Souza, Hui-Hong Zhang , Katherine J. Strissel , Marcelo A. Christoffolete, Julia Kovsan, Assaf Rudich, Fredric B. Kraemer, Antonio C. Bianco, Martin S. Obin, and Andrew S. Greenberg. 2006. Perilipin Promotes Hormone-sensitive Lipase-mediated Adipocyte Lipolysis via Phosphorylation-dependent and -independent Mechanisms. 2006 J. Biol. Chem. 281:15837-15844..

Wu Z, Huang X, Feng Y, Handschin C, Feng Y, Gullicksen PS, Bare O, Labow M, Spiegelman B, Stevenson SC. 2006. Transducer of regulated CREB-binding proteins (TORCs) induce PGC-1{alpha} transcription and mitochondrial biogenesis in muscle cells. 2006 Proc Natl Acad Sci U S A. 103(39):14379-84. .

Liu Z, Ahn JY, Liu X, Ye K. 2006. Ebp1 isoforms distinctively regulate cell survival and differentiation. 2006 Proc Natl Acad Sci U S A. 103(29):10917-22...

Hiranmoy Das, Ajay Kumar, Zhiyong Lin, Willmar D. Patino , Paul M. Hwang , Mark W. Feinberg, Pradip K. Majumder , and Mukesh K. Jain, 2006. Kruppel-like factor 2 (KLF2) regulates proinflammatory activation of monocytes. 2006 Proc Natl Acad Sci U S A. 103(17):6653-6658..

McGuire JR, Rong J, Li SH, Li XJ.. 2006 Interaction of Huntingtin-associated protein-1 with kinesin light chain: implications in intracellular trafficking in neurons. 2006 J Biol Chem.281:3552-9.

Hideaki Miyoshi, Sandra C. Souza, Hui-Hong Zhang, Katherine J. Strissel, Marcelo A. Christoffolete, Julia Kovsan||, Assaf Rudich, Fredric B. Kraeme, Antonio C. Bianco, Martin S. Obin, and Andrew S. Greenberg. 2006. Perilipin Promotes Hormone-sensitive Lipase-mediated Adipocyte Lipolysis via Phosphorylation-dependent and -independent Mechanisms. 2006 J. Biol. Chem., 281:15837-15844..

Ahn JY, Liu X, Liu Z, Pereira L, Cheng D, Peng J, Wade PA, Hamburger AW, Ye K. 2006. Nuclear Akt associates with PKC-phosphorylated Ebp1, preventing DNA fragmentation by inhibition of caspase-activated DNase. 2006 EMBO J. 25(10):2083-95..

Juan Rong, John R. McGuire, Zhi-Hui Fang, Guoqing Sheng, Ji-Yeon Shin, Shi-Hua Li, and Xiao-Jiang Li. 2006. Regulation of Intracellular Trafficking of Huntingtin-Associated Protein-1 Is Critical for TrkA Protein Levels and Neurite Outgrowth . 2006 J Neurosci.26(22): 6019-6030;.

Xu H, Wilcox D, Nguyen P, Voorbach M, Suhar T, Morgan SJ, An WF, Ge L, Green J, Wu Z, Gimeno RE, Reilly R, Jacobson PB, Collins CA, Landschulz K, Surowy T. 2006. Hepatic knockdown of mitochondrial GPAT1 in ob/ob mice improves metabolic profile. Biochem Biophys Res Commun. 349(1):439-48..

Seungil Ro, Sung Jin Hwang, Tamas Ordog, and Kenton M. Sanders. 2005. Adenovirus-based short hairpin RNA vectors containing an EGFP marker and mouse U6, human H1, or human U6 promoter. 2005 BioTechniques 38: 625-627..

Rong R, Tang X, Gutmann DH, Ye K. 2004. Neurofibromatosis 2 (NF2) tumor suppressor merlin inhibits phosphatidylinositol 3-kinase through binding to PIKE-L. 2004 Proc Natl Acad Sci U S A. 101(52):18200-5..



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WHAT IS NEW
DNA that encodes a small hairpin RNA (shRNA) molecule will be inserted into the adenoviral genome, in which the expression of shRNA is under the control of the U6 or CMV promoter.

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FAQS
Welgen technical specialists are professional researchers with laboratory and product experience. Feel free to contact us with your technical or product inquiry.

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TECH RESOURCES
Adenovirus and RNAi Services performed by Welgen Inc. have been referenced by the publications:

Sheng G et al. 2006. Hypothalamic huntingtin-associated protein 1 as a mediator of feeding behavior. Nature Medcine. 12:526-33
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