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Adenovirus
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AdenoVirus Services:
Adenovirus vectors are arguably the most widely used gene deliver system. Welgen has been providing related services to researchers in academic institutes, pharmaceutical and biotech industrials for the past five years and have constructed, amplified, and purified thousands of adenovirus. Welgen’s service has enable researchers to save time and money in their works. Currently, Welgen is providing a complete line of services relating adenovirus vectors:
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Service ID |
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Construct an adenovirus vector to express a gene (target cDNA provided by customer) |
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Construct an adenovirus vector to express a gene (target cDNA not provided by customer and will be cloned by Welgen) |
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Generation of adenovirus (adenoviral construct provided) |
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Gene silencing services- Insert a validated RNAi sequence into pQuiet vectors |
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Gene silencing services- Insert a validated RNAi sequence into an adenoviral vector |
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RNAi selection service |
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Amplification and purification of adenovirus |
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Plaque purification |
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Plaque assay to titer adenovirus |
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PCR assay for replication-competent adenovirus (RCA) |
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Assay for replication-competent adenovirus (RCA) using A549 cells |
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TCID50 (Tissue Culture Infectious Dose 50) |
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Adenoviral DNA Isolation |
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MOI Assay |
Lentivirus Services
Delivery of genes by lentiviral vectors has been well established and is currently widely used due to several advantages: Lentiviral vectors can accommodate long sequences; the vectors seem to be non-immunogenic due to the lack of viral coding sequences transfer; the vectors are able to transduce non-dividing cells; and the products can be stably expressed due to integration into the cell chromosome.
Welgen provides the following lentivirus services for research applications:
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Service ID |
Service Description |
Mini-scale Lentivirus Production |
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Standard Lentivirus Production |
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Large-scale Lentivirus Production |
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Custom Lentiviral Construction and Production |
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Complete RNAi Service using Lentiviral vector |
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Lentivirus Titering Assay |
Welgen also provides services for the construction and production of vectors based on retrovirus and avian associated virus (AAV). For vectors based on other virus, please contact Welgen.
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Service ID |
Service Description |
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Mini-scale retroviral vector production |
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Standard-scale retroviral vector production |
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Large-scale retroviral vector production |
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Custom retroviral vector construction and production |
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Complete RNAi service based on retroviral vectors |
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Retrovirus titering Assay |
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For more information about these services, please contact us at: infor@welgen.com.
Welgen also provides a variety of other services to our customers. Some of them are listed here. If you do not see the service you want, please contact Welgen to see whether we can help.
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Service ID |
Service Description |
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Virus Aliquot |
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PCR cDNA cloning (1-2 kb) |
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Subcloning a gene into expression vector (E. coli) |
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Expression and purification of protein in E. coli (1 Liter) |
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Mutagenesis |
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Vector construction and modification |
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Western blotting |
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Purify plasmid DNA |
| © 2004 Welgen, Inc. |