This vector contains the left end essential adenoviral sequences and a multiple cloning site for the insertion of a promoter along with the gene to be expressed. The plasmid may be used to evaluate gene expression in preliminary transfection experiments prior to construction of an adenoviral vector.
|
Position |
Feature |
|
2-27 |
I-Ceu I site |
|
30-404 |
Adenovirus nt 1-375 (L.ITR, enhancer, packaging signal) |
|
534-593 |
Multiple cloning site (SpeI ¨C BamHI) |
|
594-623 |
PI-Psp I site |
|
1532 |
Replication origin |
|
2693-3104 |
Cos site |
|
4371-3128 |
Tetracycline resistance (Tetr ) |