Welgen offers an improved technology to simplify and accelerate the construction of adenoviral vectors, which offer many advantages to investigators who study gene expression or gene silencing in mammalian systems. Adenoviruses have a strong safety record and recombinant adenoviral vectors have been useful tools for gene function studies as well as for human gene therapy. Recombinant adenoviruses that cannot replicate are attractive vectors for gene delivery for several reasons:
- they infect many types of mammalian cells with high efficiency
- they do not disrupt the genome of the host cell but remain in the nucleus as episomal DNA
- they provide transient, high level gene expression
- they are suitable for both in vitro and in vivo applications
Welgen has been providing the high quality services and technical supports to researchers in academic institutes, pharmaceutical and biotech industrials since 2002. Welgen has constructed, amplified, and purified thousands of adenoviruses for worldwide clients. Currently, Welgen is providing a complete line of adenoviral services.
Lentiviral and Retroviral services
Delivery of genes by lentiviral vectors has been well established and is currently widely used due to several advantages: Lentiviral vectors can accommodate long sequences; the vectors seem to be non-immunogenic due to the lack of viral coding sequences transfer; the vectors are able to transduce non-dividing cells; and the products can be stably expressed due to integration into the cell chromosome.
Welgen provides the lentiviral and retroviral services for research applications.
RNAi mediated gene silencing is a powerful tool in the study of gene function. RNAi has also become a standard approach for drug target discovery and validation. In the past 5 years, Welgen selected and validated hundreds of effective RNAi for the customers. Some research results have been published in the top journals such as Nature Medicine, PNAS etc.
Welgen currently offers several RNAi services. Read More
WHAT IS NEW
DNA that encodes a small hairpin RNA (shRNA) molecule will be inserted into the adenoviral genome, in which the expression of shRNA is under the control of the U6 or CMV promoter.
- Custom AAV Service
- RNAi Service
Welgen technical specialists are professional researchers with laboratory and product experience. Feel free to contact us with your technical or product inquiry.
Adenovirus and RNAi Services performed by Welgen Inc. have been referenced by the publications:
.Sheng G et al. 2006. Hypothalamic huntingtin-associated protein 1 as a mediator of feeding behavior. Nature Medcine. 12:526-33